Engineered split inteins
Since the 1990s, SpliceBio co-founders have been pioneering research with a family of proteins that had the potential to address this limitation: inteins. Inteins are auto-processing domains that carry out a process known as protein splicing, a multi-step biochemical reaction comprised of both the cleavage and formation of peptide bonds. However, inteins found in nature presented several limitations that rendered them unfit for the development of human therapeutics. Most recently, and building on more than 20 years of pioneering intein research characterizing the mechanism of action and structure-activity relationship of inteins and optimizing their properties, SpliceBio co-founders at Princeton University developed a new generation of inteins that overcome the limitations of natural inteins and are designed for therapeutic use: engineered split inteins.